Composite
SL-Fok_A

Part:BBa_K243007:Design

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-14)

Short Linker-Fok_a


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 505


Design Notes

The short length of the linker is useful to produce a stable connection to another part. The short distance produced by the linker reduces the distance from the protein Fok_a to the DNA when it is linked to a DNA binding protein. We had to prove the choice of distance between two parts and the distance between our protein domain Fok_a and Fok_i.
Designed according to the RFC 25. Commented GenBank file

Source

Combinated by cloning.

References